Journal: Clinical and Translational Medicine

Article Title: ZC3H13‐mediated m6A stabilization of CCND1 promotes malignant progression and is associated with poor anti‐PD‐1 response in HNSCC

doi: 10.1002/ctm2.70750

Figure Lengend Snippet: The ZC3H13/CCND1 axis remodels the HNSCC immune microenvironment and the proposed mechanistic model. (A) Gene Ontology (GO) enrichment analysis revealing biological pathways related to the negative regulation of cell activation and apoptosis. (B) Bar plot illustrating the changes in the proportion of CD4 + T cells between the Pre‐ and Post‐immunotherapy groups. (C) Boxplot comparing the quantitative CytoTRACE scores before and after immunotherapy (Pre vs. Post). (D) UMAP feature plots displaying the spatial distribution of cell differentiation states (CytoTRACE scores) in Pre‐ and Post‐treatment samples. (E) Violin plot demonstrating the significant difference in CD4+ T cell exhaustion scores between the Pre and Post treatment groups. (F) The relative mRNA expression levels of key immune checkpoint molecules (PDCD1, LAG3, CTLA4) in CCND1‐low and CCND1‐high groups were determined by RT‐qPCR. (G) Representative multiplex immunofluorescence images showing CCND1, CD4, PD‐1 and DAPI staining in mouse HNSCC tissues from control and ZC3H13‐deficient groups. Scale bar, 20 µm. (H) The quantification for the multiplex immunofluorescence (mIF) staining in the tumour microenvironment. (I) Schematic mechanistic model. Data are presented as the mean ± SD. *** p < .001.

Article Snippet: Sections were incubated with the indicated primary antibodies at room temperature for 1 h: CD4 (HY‐ P83756 , MCE, 1:500), CCND1 (HY‐ P80098 , MCE, 1:50), and PD‐1 (84651, Cell Signaling Technology, 1:100) in the optimized order of CD4, CCND1, and PD‐1.

Techniques: Activation Assay, Cell Differentiation, Expressing, Quantitative RT-PCR, Multiplex Assay, Immunofluorescence, Staining, Control

Journal: Animals : an Open Access Journal from MDPI

Article Title: An ORFV F1L mRNA Vaccine Candidate: Preparation, Immunogenicity, and Comparison with a Commercial Live Vaccine

doi: 10.3390/ani16142274

Figure Lengend Snippet: ORFV-specific antibody responses and CD4 + and CD8 + T cell responses. ( A ) Kinetics of ORFV-specific antibody responses measured by ELISA after intramuscular immunization with F1L mRNA LNPs (5, 10, 15 μg), commercial live vaccine (CV), or PBS control on days 0 and 14. ( B ) Neutralizing antibody titers (NT50) in sera collected on day 28, determined by microneutralization assay (ORFV FZ strain, 100 TCID50; BHK-21 cells). Data are mean ± SD with individual points (n = 10 per group). * p < 0.05, ** p < 0.01 vs. PBS; ns, not significant ( p > 0.05); one-way ANOVA with Dunnett’s posttest. ( C , D ) Frequency of CD4 + ( C ) and CD8 + . ( D ) T cells following the same immunization regimen as in ( A , B ). Statistical comparisons as in ( B ).

Article Snippet: PE-conjugated anti-mouse CD4, FITC-conjugated anti-mouse CD8, and PE-Cy5-conjugated anti-mouse CD3ε monoclonal antibodies were obtained from Lianke Bio (Hangzhou, China).

Techniques: Enzyme-linked Immunosorbent Assay, Control, Microneutralization Assay

Journal: iScience

Article Title: MFAP5 + synovial fibroblasts drive LOX upregulation to promote osteoarthritis progression

doi: 10.1016/j.isci.2026.116286

Figure Lengend Snippet: MFAP5 + SFs promoted collagen deposition in synovium (A) Dendrogram showing the hierarchy organization of MEGENA network modules in GSE176308 . The top layer represented the root and largest parent modules, which branched hierarchically into smaller child modules. (B) Density plots displaying c1_9 module scores across SF clusters, with color-coded density values; red indicated higher density, and blue indicated lower density. (C) Network of pathways and genes involved in the c1_9 module, where a two-point line indicated that a gene was part of a specific pathway. (D) Raincloud plot showing c1_9 score in GSE152805 . (E and F) Gene set enrichment analysis showed collagen pathways were upregulated in MFAP5 + SFs in both GSE176308 (E) and GSE152805 (F). (G) Correlation coefficients between the expression of MFAP5 and one ECM pathway score across three datasets. (H) Violin plots of ECM binding (left) and ECM organization (right) scores across SF clusters in GSE176308 . Color-coded by SF clusters. (I) Density plots of ECM binding and ECM organization scores across SF clusters in SCP469 and GSE152805 . (J) Comparison of significant ligand-receptor pairs from SF clusters to T cells in GSE152805 . (K) Immunofluorescence images of MFAP5, CD4, and vimentin colocalization in synovial tissues. Scale bars, 200 or 20 μm. Statistical significance was calculated using two-tailed Wilcoxon rank-sum test (D and H) and Spearman's rank correlation analysis (G). ∗∗∗ p < 0.001.

Article Snippet: Sections were then incubated overnight at 4 °C with primary antibodies, including vimentin (GB11192, Servicebio, China), FBN1 (860327, Zenbio, China), MFAP5 (15727-1-AP, Proteintech, China), and CD4 (GB150062-50, Servicebio, China).

Techniques: Expressing, Binding Assay, Comparison, Immunofluorescence, Two Tailed Test